Comparision study for the effect of murine's immunologic function with Bifidobacteria and E.coli's whole DNA / 中国免疫学杂志

ABSTRACT

Objective:To extract DNA from Bifidobacteria and E.coli cells and incubate DNA with IEL to observe.DNA's activation for IEL.Also plan to use the DNA to immune mice to observe the change of immunologic function,discuss the effect of Bifidobacteria and E.coli's whole DNA to murine and make a comparision study.Methods: Mice of Bifidobacteria DNA and E.coli's DNA treated groups are respectively given with DNA by muscular and subcutaneous injection. Detect the immunologic function of spleen cell, extract IEL cells and incubate them with DNA, detecting IEL activity and the production of cytokine. Results:The activities of IL 2 and NK are all significantly higer than those of in control groups (P