The relationships between structure and function of human transmembrane tumor necrosis factor-? / 中国免疫学杂志
Chinese Journal of Immunology
; (12)1986.
Article
de Zh
| WPRIM
| ID: wpr-675334
Bibliothèque responsable:
WPRO
ABSTRACT
Objective:To determine the region of human transmembrane tumor necrosis factor alpha (TM TNF?),essential for cytotoxic activity against mouse L929 cell.Methods:Single amino acid substituted TM TNF? mutant proteins(muteins) were produced by in vitro transcription linked translation techniques.An expression cDNA for TM TNF? was site directed mutagenized by recombinant PCR.7 single amino acid substituted TM TNF? muteins were generated and assayed for cytotoxic activity.Results:The cytotoxic activities of TM TNF? muteins,eg,TM TNF? -71/Lys was
Texte intégral:
1
Indice:
WPRIM
langue:
Zh
Texte intégral:
Chinese Journal of Immunology
Année:
1986
Type:
Article