mRNA expression of CD28/B7 molecules in the peripheral blood mononuclear cells of the patients with systemic lupus erythematosus and their significance / 中国免疫学杂志

ABSTRACT

Objective:To investigate the roles of CD28/B7 molecules expression in the pathogensis of systemic lupus erythematosus and their significance.Methods:Applying reverse transcription polymerase reactin(RT PCR)technique to semiquantitatively analyze CD28?B7 1 and B7 2 mRNA expression in the Peripheral Blood Mononuclear Cells(PBMC)from 35 active SLE patients and 30 cases of healthy subjects.Results:Compared with normal controls,the positive rate of CD28 expression in active SLE patients accounted for 22 86%,which was markedly lower than the normal controls(70 00%),the difference was statistically significant( P