Culture of rat skeletal muscle satellite cells and their response to stimulation by insulin-like growth factor / 中华创伤杂志

ABSTRACT

Objective To observe the proliferation and differentiation properties of rat skeletal muscle satellite cells that are dissected,cultured and purified in vitro and investigate their response to stimulation by insulin-like growth factor I(IGF-I).Methods The cells were isolated from Wistar rats by two-step enzyme digestion and purified by the Percoll density centrifugation to observe their prolif- eration and differentiation properties and draw growth curve and fusion rate curve.Then,the response of the satellite cells to stimulation by IGF-I at various concentrations was determined by using MTT meth- od.Results Plenty and purified satellite cells were obtained via these methods.Conclusion IGF- I at appropriate concentration(100 ng/ml)can promote the proliferation and differentiation of rat skele- tal muscle satellite cells.