Time-dependent change in expression of type Ⅰ collagen protein gene during the development of restenosis after arterial injury / 第二军医大学学报

ABSTRACT

Objective: To observe the alteration of type Ⅰ collagen protein gene expression after arterial injury and investigate its effect on the development of restenosis. Methods: Firstly, theexperimental carotid arterial injury rabbit model was constructed. Then, Northern blot, in situ hybridization and histomorphometric analysis were used to detect the expression of procollagen mRNA and the accumulation of collagen protein 1,2,4 weeks after injury. Results: Type Ⅰ collagen mRNA increased 1 week after injury, peaked 2 weeks later and decreased 4 weeks later. The deposition of the collagen protein account for a high percentage of space in neointima on histomorphometric analysis. Conclusion: Collagen protein may play an important role in the development of neointima and restenosis. [