EFFECTS OF VITAMIN B 1, C AND ORANGE JUICE ON HUMAN LUNG CANCER CELLS IN VITRO / 营养学报

ABSTRACT

Objective: To investigate the effects of vitamin B 1, C and orange juice on human lung cancer cell proliferation in vitro. Methods: Vitamin C degradation in culture medium was evaluated. The methods included methy thiazolyl tetrazolium (MTT) assay, colony forming assay and 3H TdR incorporation test. The final concentrations of factors in medium were orange juice (vitamin C 30,60,120 ?g/ml), vitamin C(30,60,120 ?g/ml), vitamin B 1 (0.01,0.1,1 ?g/ml). Results: (1) In MTT test, orange juice at each level had significant inhibitory effect on the growth of lung cancer cells (P