The dynamic change of ATPase activities in rat brain with propofol anesthesia / 中国药理学通报

ABSTRACT

AIM To investigate the effects of propofol on Na +, K + ATPase and Ca 2+ ATPase activities in rat brain. METHODS Forty rats were divided randomly into five groups. The animals were administered intraperitoneally (ip) propofol 100 mg?kg -1 or equal volume of normal saline (control group) respectively. These rats were immediately decapitated before (induction group) and after (anesthesia group) the disappearance of righting reflex, and when righting reflex appeared again (recovery group), and rats were completely conscious (awake group). Brain tissues were dissected on ice, then homogenized and centrifuged. Na +, K + ATPase and Ca 2+ ATPase activities were estimated by spectrophotometry. RESULTS Propofol 100 mg?kg -1 ip significantly inhibited Na +, K + ATPase and Ca 2+ ATPase activities of cortex, hippocampus and brain stem as compared with that of normal saline group ( P