Role of interleukin-9 in the pathogenesis of chronic obstructive pulmonary disease / 北京大学学报(医学版)

ABSTRACT

Objective:To study the role of interleukin 9 in the airway inflammation from patients with COPD. Methods: Induced sputum was obtained from 30 COPD patients with stable disease(group A) ,31 asthmatics patients with stable disease(group B) and 15 healthy individuals(group C). IL 9,IL 5 and IL 8 in sputum supernatants were measured by sandwich enzyme linked immunosorbent assay(ELISA) and IL 9 positive expression and quantitative analysis were conducted by Streptavidin peroxidase method and image analysis technology. Results:The levels of IL 9 in group A and B were all significantly higher than those in groups C. IL 9 positive expression mainly located in the cytoplasm of macrophages. The positive rates of IL 9 in group A and B were all significantly higher than that of group C(? 2=20.821, 19.908, P