Determination of rhien, emodin, and chrysophanol in Huangdanyinchen Granules by HPLC / 中成药

ABSTRACT

Objective:To develop a method to determine the contents of emodin, rhien and chrysophanol in Huangdanyinchen Granules. Methods: HPLC was used to determine the contents of emodin, rhien and chrysophanol in Huangdanyinchen Granules. The separation was performed on YWG ODS column with methol perchloric acid (1%) (8∶2) as a mobile phase and the wavelength of UV detector was 280nm.Results: The separating degree and the linearity of this method was well with the average recovery of rhien 97.43%, RSD= 1.90%; Emodin98.45%,RSD=1.62%;Chrysophanol 98.87%, RSD=1.50%, respectively.Conclusions:The method is simple and rapid and gives satisfactory results. It can be suitable for quality controll of Huangdanyinchen Granules.