The dynamical changes of mitogen-activated protein kinase signal transduction in rats with severe acute pancreatitis / 中华消化杂志

ABSTRACT

Objective To investigate the dynamical changes of mitogen activated protein kinase (MAPK) signal transduction in rats with severe acute pancreatitis(SAP). Methods The SAP model was induced by bili pancreatic duct infusion with 5% sterile sodium taurocholate solution. Fifty six SD rats were randomly divided into seven groups control group, 0.5 h postoperation group, 1 h group, 3 h group, 6 h group, 12 h group,24 h group. Western blot analysis was used to determine the activities of p38MAPK and c Jun N terminal kinase (JNK) in the pancreas and lung. Results In the rats of control group, the basal p38MAPK activity can be detected whereas JNK cannot. After the SAP was performed, the p38MAPK activity in pancreas increased obviously, which peaked at 3 h, but the p38MAPK activity in lung peaked at 6 h. The p38MAPK activity of pancreas and lung was significantly higher than basal activity at 24 h point. The activity of JNK was only increased at 12 h point and was not detected at 24 h point. Conclusions MAPK signal transduction pathway, in particular p38MAPK, plays an important role in the pathogenesis of SAP.