Study on quality assay of agkistrodon halys's crude venom / 中成药
Chinese Traditional Patent Medicine
; (12)1992.
Article
de Zh
| WPRIM
| ID: wpr-682255
Bibliothèque responsable:
WPRO
ABSTRACT
Objective: To establish a method for controlling the quality of Agkistrodon halys's crude venom. Methods: The method of PAGE was carried out to identificate the Agkistrodon halys' crude venom, And the protein of crude venom was also assayed by Lowry's method. Results: The Agkistrodon halys's crude venom could be identified by the method of PAGE. The determintion of protein of crude venom showed a good linear relationship in a concentration range of 25 ?g?mL -1 ~250?g?mL -1 , the regression equation was Y=0.0147+ 1.4553X , and the correlation coefficient was 0.9992. The average recovery of the content reached 100.09, RSD=1.765(n=6). Conclusion: The method is simple, feasible, accurate and available, can be used to controlling of the quality of Agkistrodon halys's crude venom.
Texte intégral:
1
Indice:
WPRIM
langue:
Zh
Texte intégral:
Chinese Traditional Patent Medicine
Année:
1992
Type:
Article