Research on Solid-state Fermentation on Rice Chaff with a Microbial Consortium / 微生物学通报

ABSTRACT

A microbial consortium of Trichoderma reesei AS3 3711, Aspergillus niger AS3 316 and Saccharomyces cerevisiae AS2 399 was constructed to decomposed rice chaff on the basis of the characters of each microorganism and the mechanism of cellulases In this experiment, rice chaff was pretreated with NaOH before fermentation so that the lignin structure of rice chaff was degraded and hemi cellulose was dissolved partly, which remove the protection of lignin and hemi cellulose on cellulose and demolish its special crystal structure After pretreatment, rice chaff can be degraded more easily with the microbial consortium The optimal technical paths and technological methods were achieved for intenerating rice chaff with the microbial consortium perfectly through orthogonal experiment According to the technological methods, some experiments were done at 30℃ with pH 4 5 It was found that the highest filter paper enzyme activity (FPA) was 5 64U/g and the ratio of cellulose degradation (RCD) was 28 05%