Preparation and testing of hepatitis C core antigen ELISA reagent / 中国输血杂志
Chinese Journal of Blood Transfusion
; (12)2002.
Article
Dans Zh
| WPRIM
| ID: wpr-684588
Responsable en Bibliothèque :
WPRO
ABSTRACT
Objective To establish a sandwich enzyme-linked immunosorbent (ELISA) method for early diagnosis of hepatitis C. Methods Immunization of Balb/c mice with hepatitis C core antigen were prepared by genetic engineering. Mouse monoclonal antibodies (McAb) to anti-HCV-core Ag were obtained. A sandwich ELISA kit for detecting HCV-core Ag was developed by using four strains of anti-HCV-core Ag McAb. One hundred and twenty five serum specimens with increased ALT but negative for anti-HCV, anti-HIV, and anti-Tp tests were tested. Results Nine of the 125 specimens were positive for HCV-core Ag. Conclusion The double sandwich ELISA kits for detecting HCV-core Ag may be useful for the early diagnosis of hepatitis C .
Texte intégral:
1
Indice:
WPRIM
Type d'étude:
Screening_studies
langue:
Zh
Texte intégral:
Chinese Journal of Blood Transfusion
Année:
2002
Type:
Article