Cloning, Sequencing of Suaeda heteroptera kitag CMO cDNA and Construction of its Recombinant Plant Expression Vector / 中国生物工程杂志
China Biotechnology
; (12)2006.
Article
Dans Zh
| WPRIM
| ID: wpr-685042
Responsable en Bibliothèque :
WPRO
ABSTRACT
Total RNA was extracted from leaf of Suaeda hetroptera kitag, then the CMO ( choline monooxygenase) cDNA was amplified using the reverse transcriptase polymerase chain reaction ( RT-PCR) method and cloned into pMD-T-simple vector. The positive clones from the Blue/White Screen were sequenced. After confirming its validity, the CMO gene fragment was cloned into pBI121 vector. Double enzyme restriction and PCR analysis indicated that the pBI121/CMO recombinant plasmid was successfully constructed.
Texte intégral:
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Indice:
WPRIM
langue:
Zh
Texte intégral:
China Biotechnology
Année:
2006
Type:
Article