Effect of IKK2 Inhibitor LY2409881 on Diffuse Large B-Cell Lymphoma Cell Apoptosis and Its Mechanism / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1086-1092, 2018.
Article
Dans Zh
| WPRIM
| ID: wpr-689524
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects and its potential mechanism of IKK2 inhibitor LY2409881 on proliferation, cell cycle and apoptosis of diffuse large B-cell lymphoma (DLBCL) cell lines.</p><p><b>METHODS</b>CCK8 assay was used to detect the effect of LY2409881 on proliferation of DLBCL cell lines; Flow cytometry was used to analyze cell cycle; Western blot was used to analyze apoptosis and its potential mechanism.</p><p><b>RESULTS</b>LY2409881 inhibited the proliferation of multiple DLBCL cell lines obviously, and caused G cell arrest. Furtherly, LY2409881 inhibited the expression of c-FLIP, induced the activation of DR4 and caspase 8. Meanwhile, LY2409881 induced the expression of pro-apoptotic protein BAX and inhibited the expression of anti-apoptotic protein MCL-1 and BCL-2.</p><p><b>CONCLUSION</b>LY2409881 inhibits the proliferation of DLBCL cell lines, causes G cell arrest and induces apoptosis via the endogenous and exogenous apoptotic pathways.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pyrimidines
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Thiophènes
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Lymphome B diffus à grandes cellules
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Apoptose
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Lignée cellulaire tumorale
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Prolifération cellulaire
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I-kappa B Kinase
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Journal of Experimental Hematology
Année:
2018
Type:
Article