Study on XRCC1 gene polymorphisms and EBV infection in nasopharyngeal carcinoma and underlying mechanism / 中国耳鼻咽喉头颈外科

ABSTRACT

OBJECTIVE To investigate the relationship between genetic polymorphim of X-ray repair cross complementing gene 1(XRCC1) and EBV infection in NPC cell.METHODS NPC CNE cell strain was divided into different groups depending on its XRCC1 genetic polymorphism,and EBV was transferred into different groups.Characteristics of cells and XRCC1 protein were detected among different groups before and after EBV transferring.RESULTS There was no difference in cell characteristics and XRCC1 protein level among different genotypes in NPC CNE cells strain.After EBV transferring,there was an increasing in cell malignancy in NPC cells,including control group,wild type group,and 194,280,399 mutant genotype groups,and the differences were statistically significant(P>0.05).There was no difference in XRCC1 protein level before and after EBV transferring.Compared with other groups,the increasing in cell malignancy was less in the 194 mutant genotype group before and after EBV transferring,especially in cell migration and proliferation.There were no difference in the increasing of cell malignancy and XRCC1 protein level among control group,wild type and 280,399 mutant genotypes of NPC CNE cells before and after EBV transferring.CONCLUSION The results suggest that EBV transferring can raise the carcinogenicity of NPC cell.After EBV transferring,the 194 mutant genotype is associated with a reduced carcinogenicity of NPC cells compared with other genotypes.