Apply of Genotype MTBDRplus on detecting the resistance of Mycobacterium tuberculosis / 国际检验医学杂志

ABSTRACT

Objective To investigate the adaptability of Genotype M TBDRplus on detecting the resistance of Mycobacterium tuberculosis.Methods Analyze and compare the results of Proportional drug sensitivity test and Genotype M TBDRplus detection in 479 strains of Mycobacterium tuberculosis from Tuberculosis Pre-vention and Control in Changping District from 2013 to 2015.Results A total of 434 strains in the 479 strains were detected as Mycobacterium tuberculosis by both of the two tests.The sensitivity and specificity of Geno-type M TBDRplus on detecting rifampiain(RIF)in the 434 stains of Mycobacterium tuberculosis were 96.55%(28/29)and 99.51%(403/405),the compliance rate of the two methods was 99.31%(431/434),and the mu-tation site was mainly S531L.The sensitivity and specificity of isohiazid(INH)were 88.14%(52/59)and 94.93%(356/375),the compliance rate of the two methods was 94.01%(408/434),and mutation sites were mainly C15T and S315T1.Conclusion Genotype M TBDRplus detection could provide the results of RIF and IN H resistance,and it is an effective supplement to the traditional proportional drug sensitivity method.