Synthetic and Adenovirus Delivered Small Interference RNA Pools Targeting Conserved Regions of Foot-and-Mouth Disease Virus
Journal of Bacteriology and Virology
;
: 199-206, 2010.
Article
Dans Anglais
| WPRIM
| ID: wpr-69383
ABSTRACT
Foot-and-mouth disease (FMD) is an economically significant animal disease because of the speed of its transmission. Routine vaccination may not be effective for early protection in an outbreak situation. Small interfering RNA (siRNA) can be used as a rapid, effective, and an alternative antiviral approach. In this study, we screened 15 synthetic siRNAs to inhibit FMD virus replication in IBRS-2 cells and selected 10 siRNA sequences. Furthermore, we produced 7 adenoviruses expressing shRNA targeting conserved regions of FMDV, such as a leader sequence and nonstructural protein regions, and showed their antiviral effects. We compared the antiviral effects among them and compared between synthetic siRNAs and adenovirus-delivered siRNAs. In particular, the most efficient siRNA, 3C2, was the conserved sequence in the O, A, Asia 1, and C serotypes of FMDV and was located in the predicted loop structure. The pool of sequences including 3C2 and recombinant adenoviruses could be applied for multiple siRNAs and protection in a broad range of cells and animals.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Asie
/
Réplication virale
/
ARN
/
Adenoviridae
/
Vaccination
/
Séquence conservée
/
Virus de la fièvre aphteuse
/
Petit ARN interférent
/
Fièvre aphteuse
Limites du sujet:
Animaux
Pays comme sujet:
Asie
langue:
Anglais
Texte intégral:
Journal of Bacteriology and Virology
Année:
2010
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS