Analysis of inter-species cross-contamination in 160 non-human cell lines / 基础医学与临床
Basic & Clinical Medicine
;
(12): 632-637, 2018.
Article
Dans Chinois
| WPRIM
| ID: wpr-693955
ABSTRACT
Objective To analyze interspecies cross-contamination of 160 non-human cell lines.Methods One hundred and sixty common non-human cell lines were collected and their species were identified by PCR.For the suspicious cells,chromosome analysis was further used to confirm their species.Results Six in 160 non-human cell lines were cross-contaminated.A rat cell line was mixed by a human cell line,and 5 were totally cross-con-taminated,and were indentified as wrong species.Conclusions Species identification is an indispensable part of cell quality control.Each cell line should undergo a full QA(Quality Assurance)assessment before it is used for research.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Basic & Clinical Medicine
Année:
2018
Type:
Article
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