Serum miRNA-767-3p level in patients with colorectal cancer and its diagnostic value / 复旦学报（医学版）

ABSTRACT

Objective To assess potential diagnostic value of miRNA-767-3p in colorectal cancer (CRC).Methods The serum miRNA-767-3p levels in CRC patients,colorectal adenoma patients and healthy people were determined using real time quantitative PCR (qRT-PCR).The concentration of carcino embryonic antigen (CEA) was detected via chemiluminescent immunoassay (CLIA) in above three groups.Receiver operating curves (ROC) were established to evaluate the diagnostic efficiencies of miRNA-767-3p and combination with CEA in CRC patients.Results The levels of miRNA-767-3p were declined in CRC patients,colorectal adenoma patients and healthy people,respectively.ROC curves illustrated that the area under ROC curve (AUC) of miRNA-767-3p was up to 0.770 (95％CI0.698-0.842),with sensitivity of 80％ and the specificity of 68％ in CRC diagnosis.Moreover,a higher AUC up to 0.862 (95％CI0.806-0.918) with the sensitivity of 73％ and the specificity of 84％ was achieved when miRNA-767-3p and CEA were combined.Conclusions Serum miRNA-767-3p could be considered as an independent diagnostic marker of CRC,and miRNA-767-3p combined with CEA would increase the detective quantum efficiency of CRC.