Expression of SRC1 in gastric cancer and its prognosis / 实用肿瘤学杂志

ABSTRACT

Objective The objective of this study was to investigate the expression of SRC1 protein in gastric cancer,and its correlation with clinicopathological parameters and prognosis.Methods Thirty-six gastric cancer tissues and matched paracancerous tissues were collected.The expressions of SRC1 at levels of mRNA and protein were detected by qRT-PCR and Western blot.The ex-pression of SRC1 protein in 286 cases of gastric cancer was detected by immunohistochemistry.The relationship between the expres-sion of SRC1 protein and the clinicopathologic parameters of gastric cancer and the prognosis of the patients were analyzed.Results The expression of SRC1 at levels of mRNA and protein were significantly lower in gastric cancer tissues than that in the normal gastric tissues(P=0.004).There was no significant correlation between the expression of SRC1 protein in gastric cancer tissues and the clin-icopathological parameters of gastric cancer patients.Kaplan-Meier survival curve analysis showed that the 5-year survival rate of SRC1 overexpressing group was significantly higher than that in the low expression group(P=0.009).Cox regression analysis showed that the low expression of SRC1,tumor invasion of T4a~T4b,lymph node metastasis N,and distant metastasis M1 were independent prognosis predictors of total survival time(P<0.05 or P<0.001).The low expression of SRC1 protein was associated with poor prog-nosis in gastric cancer.Conclusion The expression of SRC1 protein is low in gastric cancer.The SRC1 expression may be an inde-pendent prognostic factor for gastric cancer.The overexpression of SRC1 may be related to the development of gastric cancer,which may serve as a marker of tumor suppressor gene and prognosis of gastric cancer patients.