Osthol effects on the proliferation and differentiation of osteoblasts based on endoplasmic reticulum stress / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 1817-1822, 2018.
Article
Dans Chinois
| WPRIM
| ID: wpr-698619
ABSTRACT
BACKGROUND:
Osthol has been reported to promote osteogenesis by increasing osteoblast proliferation, but the anti-osteoporosis mechanism underlying osthol is poorly understood.OBJECTIVE:
To observe the effect of osthol on the proliferation and differentiation of rat osteoblasts in vitro and to explore its mechanism of anti-osteoporosis effect.METHODS:
Rat osteoblasts were isolated by secondary enzyme digestion and identified by alkaline phosphatase staining and mineralized nodule staining. There were five groups blank control, solvent control, β-estradiol as well as low-, medium- and high-dose osthol groups. Cell proliferation was detected by cell counting kit-8 assay, and cell differentiation was evaluated by detection of alkaline phosphatase and mineralized nodule staining. The expression levels of GRP78, PDI and CHOP were detected by western blot assay. RESULTS ANDCONCLUSION:
Osthol at the concentrations of 1x10-4and 1x10-5mol/L could inhibit osteoblast proliferation. 1x10-4mol/L osthol could increase the activity of alkaline phosphatase in osteoblasts and enhance osteoblastic mineralization. Meanwhile, 1x10-4mol/L osthol was able to down-regulate the expression level of GRP78 and up-regulate the expression levels of PDI and CHOP. To conclude, osthol can promote osteoblast differentiation and inhibit osteoblast proliferation probably by endoplasmic reticulum stress.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Chinese Journal of Tissue Engineering Research
Année:
2018
Type:
Article
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