Clinical significance of the expression of serum microRNA-21, microRNA-122 and microRNA-145 in patients with primary hepatocellular carcinoma / 新乡医学院学报

ABSTRACT

Objective To investigate the clinical significance of the expression of serum microRNA-21 (miR-21),microRNA-122(miR-122) and microRNA-145 (miR-145) in patients with primary hepatocellular carcinoma(HCC).Methods A total of 100 patients with HCC who hospitalized in the Central Hospital of Tongchuan Mining Bureau from September 2014 to September 2016 were selected as HCC group,and 100 healthy subjects were selected as the control group.The expression of serum miR-21,miR-122 and miR-145 was detected by real-time quantitative fluorescence polymerase chain reaction.The relationship between the expression of serum miR-21,miR-122,miR-145 and the clinical characteristics of HCC patients was analyzed.Results The expression of serum miR-21 and miR-122 in HCC group was significantly higher than that in the control group(P ＜0.05),and the expression of serum miR-145 in HCC group was significantly lower than that in the control group (P ＜0.05).The expression of serum miR-21,miR-122 and miR-145 in HCC patients was significantly correlated with histological differentiation,clinical stage,tumor size and liver cirrhosis (P ＜ 0.05),but the expression of serum miR-21,miR-122 and miR-145 in HCC patients was not correlated with gender,age,hepatitis B virus antigen and hepatitis C virus antibody(P ＜0.05).The expression of serum miR-21 and miR-122 in the patients with poorly differentiated tissue,high clinical stage,tumor diameter ≥5 cm and liver cirrhosis was significantly higher than that in the patients with medium and high differentiated tissue,low clinical staging,tumor diameter ＜ 5 cm and no cirrhosis (P ＜ 0.05).The expression of serum miR-21 and miR-122 after operation was significantly lower than that before operation(P ＜ 0.05),and the expression of miR-145 after operation was significantly higher than that before operation in HCC patients(P ＜ 0.05).Conclusion The up-regulated expression of miR21 and miR-122 and the down-regulation of miR-145 may be related to the occurrence and development of HCC.The combined detection of serum miR-21,miR-122 and miR-145 is helpful for the diagnosis and evaluation of HCC.