CHK1 inhibitor SCH900776 suppresses proliferation and migration of U251 cells / 中国病理生理杂志

ABSTRACT

AIM:To investigate the effect of SCH900776, an inhibitor of checkpoint kinase 1(CHK1), on the proliferation and migration abilities of human glioma U 251 cells.METHODS:The cell viability was detected by MTT assay,and cell proliferation was determined by cell colony formation assay.Cell cycle distribution was analyzed by flow cy-tometry.Wound healing assay was used to determine the cell migration ability.The protein levels were determined by Western blot.RESULTS: SCH900776 inhibited the growth of U251 cells in a dose-dependent manner(P <0.05). SCH900776 treatment substantially induced U251 cell cycle arrest in S and G 2/M phases by decreasing the level of cell di-vision cycle protein 2(Cdc2)and p-Cdc2.Moreover,SCH900776 inhibited the cell migration.Western blot results indi-cated that SCH900776 increased the phosphorylation level of p 38 MAPK and inhibited the activation of Akt.CONCLU-SION:SCH900776 inhibits the proliferation and migration abilities in human U 251 cells by promoting the phosphorylation of p38 MAPK and suppressing the activation of Akt.