Effects of lead exposure on mitochondria of osteoblast MC3T3-E1 / 局解手术学杂志

ABSTRACT

Objective To evaluate the effect of lead exposure on mitochondria of osteoblasts MC3T3-E1. Methods Osteoblasts MC3T3-E1 with different concentrations(0,1,10,100μmol/L)of lead acetate[Pb(Ac) 2] solution were incubated for 24h. After that,the degree of depolarization of mitochondrial membrane potential,the amount of ATP synthesis,cytochrome c content in cytoplasm and mitochon-dria and Caspase-3 activity were measured. In addition,the morphological changes of mitochondria were observed by transmission electron mi-croscope. Results Lead exposure can cause the decrease of mitochondrial membrane potential,the decrease of ATP synthesis in osteoblasts,Ithe release of cytochrome c from mitochondria to cytoplasm and the increase of Caspase-3 activity in MC3T3-E1 cells. All results are significantly dose-dependent. n addition,through electron microscopy observation,it was found that the mitochondria in exposure of 100 μmol/L lead acetate swelled obviously. Conclusion Lead exposure can cause the functional and structural damage of MC3T3-E1 mitochondria and thus result in apoptosis.