Regulation of the apoptosis on the rat granulosa cell: effects of EGF, TGF-alpha / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
;
: 2439-2446, 2004.
Article
Dans Coréen
| WPRIM
| ID: wpr-70287
ABSTRACT
OBJECTIVE:
To evaluate the effect of the epidermal growth factor (EGF) and the transforming growth factor-alpha (TGF-alpha) on the apoptosis of the rat granulosa cells.METHODS:
For this study, granulosa cells were isolated from female rats, and granulosa cells were cultured in the presence or absence of different autocrine/paracrine factors such as EGF and TGF-alpha. In order to investigate the apoptotic process, the number of apoptotic cells were counted using Annexin V staining via flow cytometry. In addition, the expression levels of the Bcl-2, ICE and BAX genes were checked.RESULTS:
c-AMP induced apoptosis was inhibited neither by EGF nor by TGF-alpha, but FSH induced apoptosis was inhibited by EGF and TGF-alpha. Both EGF and TGF-alpha decreased the rate of apoptosis in the serum free cultured media in a time dependent manner by inducing survival gene expression such as Bcl-2 and suppressing death gene expression such as ICE and BAX.CONCLUSION:
EGF and TGF-alpha can be used to decrease the apoptotic rate of the rat granulosa cells. It is hoped to conduct a similar study on the function of these growth factors in the human granulosa cells, and to use EGF and TGF-alpha for reducing the apoptosis rate of the human granulosa cells in IVF.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Facteurs de croissance transformants
/
Expression des gènes
/
Facteur de croissance transformant alpha
/
Apoptose
/
Annexine A5
/
Protéines et peptides de signalisation intercellulaire
/
Facteur de croissance épidermique
/
Espoir
/
Cytométrie en flux
/
Cellules de la granulosa
Limites du sujet:
Animaux
/
Femelle
/
Humains
langue:
Coréen
Texte intégral:
Korean Journal of Obstetrics and Gynecology
Année:
2004
Type:
Article
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