Study on rapid recognition technique of schistosome susceptibility water bodyⅠAnimal skin making and effect assessment / 中国血吸虫病防治杂志

ABSTRACT

Objective To develop a bionic animal skin and evaluate its detective effect of Schistosoma japonicum cercariae so as to provide a new technique for the monitoring of the schistosome susceptibility water body.Methods A bionic animal skin was made with pigskin and the experiments were carried out for evaluating its detective effects of S.japonicum cercariae in labo-ratory and simulate fields,and at the same time,the sentinel mouse method was used as the control.Results In the laborato-ry,the cercariae were found in 10-,30-,60-cercaria subgroups in the bionic animal skin group after 1 and 2 hours,but the cer-cariae were found only in the 60-cercaria subgroup in the sentinel mouse group.In the simulate fields,in the water body with 5 schistosome-infected Oncomelania hupensis snails,the cercariae were found in 2 bionic animal skin devices(2/4)and 3 bionic animal skin devices(3/4)after 2 hours and 4 hours respectively,and in the water body with 10 schistosome-infected O.hupensis snails,the cercariae were found in 4 bionic animal skin devices(4/4)and 3 bionic animal skin devices(3/4)after 2 hours and 4 hours respectively in the bionic animal skin group.In the the sentinel mouse group,in the above-mentioned water bodies(with 5 or 10 schistosome-infected O.hupensis snails),the cercariae were found in 2 sentinel mice(2/4)and 1 sentinel mice(1/4)af-ter 2 hours and 4 hours respectively;and the cercariae were found in 2 sentinel mice(2/4)and 3 sentinel mice(3/4)after 2 hours and 4 hours respectively.Conclusion The bionic animal skin device can be used to detect the S.japonicum cercariae, and its sensibility and efficiency are superior to the traditional sentinel mouse method.