Simultaneous Determination of 4 Components in Shenbai Shuxin Granules by HPLC / 中国药房
China Pharmacy
; (12): 4265-4268, 2017.
Article
de Zh
| WPRIM
| ID: wpr-704423
Bibliothèque responsable:
WPRO
ABSTRACT
OBJECTIVE:To establish a method for simultaneous determination of sodium danshensu,protocatechuic aldehyde,salvianolic acid B and phellodendrine hydrochloride in Shenbai shuxin granules.METHODS:HPLC method was adopted.The separation was performed on Waters sunfire-C18 column with mobile phase consisted of acetonitrile-0.05 % trifluoroacetic acid (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set at 288 nm,and the column temperature was 30 ℃.The sample size was 5 μL.RESULTS:The linear ranges of sodium danshensu,protocatechuic aldehyde,salvianolic acid B and phellodendrine hydrochloride were 0.040 01-1.600 46 μg(r=0.999 9),0.013 84-0.553 7 μg(r=0.999 9),0.049 32-1.972 94 μg(r=0.999 6),0.014 46-0.578 6 μg(r=0.999 8).The limits of quantitation were 7.68,2.66,4.74,1.38 ng,and the limits of detection were 1.92,0.66,2.36,0.69 ng,respectively.RSDs of precision,stability and reproducibility tests were all lower than 2%.The recoveries were 98.846%-100.762% (RSD=0.77%,n=6),96.632%-99.463% (RSD=0.98%,n=6),98.541%-100.432% (RSD=0.82 %,n =6),98.607 %-101.521% (RSD =1.11%,n =6),respectively.CONCLUSIONS:The method is simple,accurate and precise.It can be used for 4 components in Shenbai shuxin granules.
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WPRIM
langue:
Zh
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China Pharmacy
Année:
2017
Type:
Article