Simultaneous determination of four constituents in Jinlan Xiaoyan Tablets by HPLC / 中成药

ABSTRACT

AIM To establish an HPLC method for the simultaneous content determination of four constituents in Jinlan Xiaoyan Tablets (Lonicerae japonicae Flos,Paeoniae Radix Rubra,Salviae miltiorrhizae Radix et Rhizoma,etc.).METHODS The analysis of 80％ methanol extract of this drug was performed on a 30 ℃ thermostatic Wondasil C1s column (250 mm ×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1％ phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 327,230,286 and 250 nm.RESULTS Chlorogenic acid,paeoniflorin,salvianolic acid B and glycyrrhizic acid showed good linear relationships within the ranges of 46.48-464.84 μg/mL (r =0.999 5),20.28-202.75 μg/mL (r=0.999 1),12.44-124.35 μg/mL (r =0.999 6) and 15.09-150.89 μg/mL (r =0.999 9),whose average recoveries were 99.30％,99.10％,99.38％ and 100.27％ with the RSDs of 1.35％,1.37％,1.28％ and 0.86％,respectively.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Jinlan Xiaoyan Tablets.