Simultaneous determination of nine constituents in Jiangtangjia Tablets by HPLC / 中成药

ABSTRACT

AIM To establish an HPLC method for the simultaneous content determination of catalpol,acteoside,jionoside B1,martinoside,calycosin-7-O-β-D-glucoside,ononin,calycosin,formononetin and heterophyllin B in Jiangtangjia Tablets (Rehmanniae Radix,Astragali Radix,Pseudostellariae Radix,etc.).METHODS The analysis of methanol extract of this drug was performed on a 30 ℃ thermostatic Agilent TC-C18 column (4.6 mm × 250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1％ glacial acetic acid flowing at 0.9 mL/min in a gradient elution manner,and the detection wavelengths were set at 203,210,254,330 nm.RESULTS Nine constituents showed good linear relationships within their own ranges (r ＞ 0.999 0),whose average recoveries were 96.86％-100.30％ with the RSDs of 0.74％-1.68％.CONCLUSION This stable and reliable method can be used for the quality control of Jiangtangjia Tablets.