Comparison of Four Human Papillomavirus Genotyping Methods: Next-generation Sequencing, INNO-LiPA, Electrochemical DNA Chip, and Nested-PCR
Annals of Laboratory Medicine
;
: 139-146, 2018.
Article
Dans Anglais
| WPRIM
| ID: wpr-713685
ABSTRACT
BACKGROUND:
Human papillomavirus (HPV) infection causes cervical cancer, thus necessitating early detection by screening. Rapid and accurate HPV genotyping is crucial both for the assessment of patients with HPV infection and for surveillance studies.METHODS:
Fifty-eight cervicovaginal samples were tested for HPV genotypes using four methods in parallel nested-PCR followed by conventional sequencing, INNO-LiPA, electrochemical DNA chip, and next-generation sequencing (NGS).RESULTS:
Seven HPV genotypes (16, 18, 31, 33, 45, 56, and 58) were identified by all four methods. Nineteen HPV genotypes were detected by NGS, but not by nested-PCR, INNO-LiPA, or electrochemical DNA chip.CONCLUSIONS:
Although NGS is relatively expensive and complex, it may serve as a sensitive HPV genotyping method. Because of its highly sensitive detection of multiple HPV genotypes, NGS may serve as an alternative for diagnostic HPV genotyping in certain situations.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
ADN
/
Tumeurs du col de l'utérus
/
Dépistage de masse
/
Séquençage par oligonucléotides en batterie
/
Génotype
/
Méthodes
Type d'étude:
Étude de dépistage
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Annals of Laboratory Medicine
Année:
2018
Type:
Article
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