Alginate-Catechol Cross-Linking Interferes with Insulin Secretion Capacity in Isolated Murine Islet Cells
Diabetes & Metabolism Journal
;
: 164-168, 2018.
Article
Dans Anglais
| WPRIM
| ID: wpr-714101
ABSTRACT
Over the past three decades, human pancreatic islet isolation and transplantation techniques have developed as a routine clinical procedure for selected patients with type 1 diabetes mellitus. However, due to the donor shortage and required chronic systemic immunosuppression, the widespread application of islet transplantation is limited. To overcome these limitations, providing a physical barrier to transplanted islet cells with encapsulating biomaterial has emerged as a promising approach to enhance engraftment and promote islet survival post-transplantation. Alginate has been considered to be a reliable biomaterial, as it enhances islet survival and does not hamper hormone secretion. Alginate-catechol (Al-CA) hydrogel was reported to provide high mechanical strength and chemical stability without deformation over a wide range of pH values. In this study, we, demonstrated, for the first time in the literature, that encapsulation of murine pancreatic islet cells with Al-CA hydrogel does not induce cytotoxicity ex vivo for an extended period; however, it does markedly abate glucose-stimulated insulin secretion. Catechol should not be considered as a constituent for alginate gelation for encapsulating islet cells in the application of islet transplantation.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Accessibilité architecturale
/
Donneurs de tissus
/
Téméfos
/
Transplantation d'ilots de Langerhans
/
Ilots pancréatiques
/
Immunosuppression thérapeutique
/
Hydrogels
/
Diabète de type 1
/
Concentration en ions d'hydrogène
/
Insuline
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Diabetes & Metabolism Journal
Année:
2018
Type:
Article
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