Immunophenotypic Characterization and Quantification of Neoplastic Bone Marrow Plasma Cells by Multiparametric Flow Cytometry and Its Clinical Significance in Korean Myeloma Patients
Journal of Korean Medical Science
; : 542-549, 2013.
Article
de En
| WPRIM
| ID: wpr-71535
Bibliothèque responsable:
WPRO
ABSTRACT
Multiparametric flow cytometry (MFC) allows discrimination between normal and neoplastic plasma cells (NeoPCs) within the bone marrow plasma cell (BMPC) compartment. This study sought to characterize immunophenotypes and quantitate the proportion of NeoPCs in BMPCs to diagnose plasma cell myeoma (PCM) and evaluate the prognostic impact of this method. We analyzed the MFC data of the bone marrow aspirates of 76 patients with PCM and 33 patients with reactive plasmacytosis. MFC analysis was performed using three combinations: CD38/CD138/-/CD45; CD56/CD20/CD138/CD19; and CD27/CD28/CD138/CD117. The plasma cells of patients with reactive plasmacytosis demonstrated normal immunophenotypic patterns. Aberrant marker expression was observed in NeoPCs, with negative CD19 expression observed in 100% of cases, CD56+ in 73.7%, CD117+ in 15.2%, CD27- in 10.5%, CD20+ in 9.2%, and CD28+ in 1.3%. In PCM patients, more than 20% of NeoPCs/BMPCs were significantly associated with factors suggestive of poor clinical outcomes. Patients who were CD27- or CD56+/CD27-, demonstrated shorter overall survival than patients of other CD56/CD27 combinations. Our results support the clinical value of immunophenotyping and quantifying NeoPCs in PCM patients. This strategy could help to reveal poor prognostic categories and delineate surrogate markers for risk stratification in PCM patients.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pronostic
/
Cellules souches tumorales
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Cellules de la moelle osseuse
/
Facteurs de risque
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Immunophénotypage
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Antigènes CD27
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Antigènes CD56
/
Asiatiques
/
Estimation de Kaplan-Meier
/
République de Corée
Type d'étude:
Etiology_studies
/
Prognostic_studies
/
Risk_factors_studies
Limites du sujet:
Adult
/
Aged
/
Aged80
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Female
/
Humans
/
Male
Pays comme sujet:
Asia
langue:
En
Texte intégral:
Journal of Korean Medical Science
Année:
2013
Type:
Article