Your browser doesn't support javascript.
loading
Early fragment removal on in vitro fertilization day 2 significantly improves the subsequent development and clinical outcomes of fragmented human embryos / 대한생식의학회지
Clinical and Experimental Reproductive Medicine ; : 122-128, 2018.
Article Dans Anglais | WPRIM | ID: wpr-716902
ABSTRACT

OBJECTIVE:

To determine whether fragment removal on in vitro fertilization (IVF) day 2 improved the subsequent development and pregnancy outcomes of fragmented embryos compared to similar-grade embryos without fragment removal.

METHODS:

This study was a retrospective analysis involving 191 IVF cycles in which all embryos had over 10% fragmentation (grade 3 or 4) on day 2 of the IVF-embryo transfer cycle from March 2015 to December 2017. IVF cycles were divided into the fragment removal group (n=87) and the no fragment removal group (n=104) as a control cohort. Before fragment removal, embryos with fragmentation on day 2 were incubated in Ca2+- and Mg2+-free biopsy medium under paraffin oil for 30 minutes. Microsurgical fragment removal was performed with later-assisted hatching and a handmade suction micropipette that had an outer diameter of 30 µm.

RESULTS:

There were no significant differences in the characteristics of the patients between the control and the fragment removal groups. After fragment removal and subsequent in vitro culture for 24 hours, the number of blastomeres (7.1±1.7 vs. 6.9±1.6) was comparable between the transferred embryos in the two groups, but the morphological grade of the embryos in the fragment removal group (1.9±0.7) was significantly higher than that of the control group (3.1±0.5, p < 0.01). The clinical pregnancy (43.7%) and implantation rates (25.8%) in the fragment removal group were significantly higher than those in the control group (28.8% and 14.0%, respectively; p < 0.05).

CONCLUSION:

Early fragment removal on day 2 significantly improved the subsequent development and pregnancy outcomes of fragmented embryos.
Sujets)

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Paraffine / Aspiration (technique) / Biopsie / Blastomères / Techniques in vitro / Issue de la grossesse / Fécondation in vitro / Études rétrospectives / Études de cohortes / Structures de l&apos;embryon Type d'étude: Etude d'étiologie / Etude d'incidence / Étude observationnelle / Facteurs de risque Limites du sujet: Femelle / Humains / Grossesse langue: Anglais Texte intégral: Clinical and Experimental Reproductive Medicine Année: 2018 Type: Article

Documents relatifs à ce sujet

MEDLINE

...
LILACS

LIS

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Paraffine / Aspiration (technique) / Biopsie / Blastomères / Techniques in vitro / Issue de la grossesse / Fécondation in vitro / Études rétrospectives / Études de cohortes / Structures de l&apos;embryon Type d'étude: Etude d'étiologie / Etude d'incidence / Étude observationnelle / Facteurs de risque Limites du sujet: Femelle / Humains / Grossesse langue: Anglais Texte intégral: Clinical and Experimental Reproductive Medicine Année: 2018 Type: Article