Your browser doesn't support javascript.
loading
Use of serology and polymerase chain reaction to detect atypical respiratory pathogens during acute exacerbation of chronic obstructive pulmonary disease
The Korean Journal of Internal Medicine ; : 941-951, 2018.
Article Dans Anglais | WPRIM | ID: wpr-717188
ABSTRACT
BACKGROUND/

AIMS:

To use serological and multiplex polymerase chain reaction (PCR) assays to examine sputum samples from patients experiencing acute exacerbation of chronic obstructive pulmonary disease (AECOPD) for the presence of atypical pathogens, including Mycoplasma pneumoniae, Chlamydia pneumoniae, and Legionella pneumophila.

METHODS:

From September 2012 to February 2014, 341 patients with AECOPD attending outpatient clinics were enrolled as part of a randomized, double-blind, multicenter study. A commercial enzyme-linked immunosorbent assay was used to measure serum immunoglobulin M (IgM) and IgG antibody titers on the first day of the study and at 36 days post-enrollment. Multiplex PCR was used to test sputum samples for the presence of atypical pathogens. A urinary antigen test for L. pneumophila was performed on the first day.

RESULTS:

Nineteen patients (5.6%) showed serological evidence of acute infection with M. pneumoniae. Also, one and seven patients (2%) showed serological evidence of acute infection with C. pneumoniae and L. pneumophila, respectively. All DNA samples were negative for M. pneumoniae, C. pneumoniae, and L. pneumophila according to PCR. Only one urine sample was positive for L. pneumophila antigen, but serologic evidence was lacking.

CONCLUSIONS:

Serological testing suggested that infection by atypical pathogens during AECOPD was relatively uncommon. In addition, PCR provided no direct evidence of infection by atypical pathogens. Thus, atypical pathogens may not be a major cause of AECOPD in South Korea.
Sujets)

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Pneumopathie infectieuse / Pneumopathie à mycoplasmes / Expectoration / ADN / Immunoglobuline G / Immunoglobuline M / Test ELISA / Tests sérologiques / Réaction de polymérisation en chaîne / Legionella pneumophila Type d'étude: Essai clinique contrôlé / Etude diagnostique Limites du sujet: Humains Pays comme sujet: Asie langue: Anglais Texte intégral: The Korean Journal of Internal Medicine Année: 2018 Type: Article

Documents relatifs à ce sujet

MEDLINE

...
LILACS

LIS

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Pneumopathie infectieuse / Pneumopathie à mycoplasmes / Expectoration / ADN / Immunoglobuline G / Immunoglobuline M / Test ELISA / Tests sérologiques / Réaction de polymérisation en chaîne / Legionella pneumophila Type d'étude: Essai clinique contrôlé / Etude diagnostique Limites du sujet: Humains Pays comme sujet: Asie langue: Anglais Texte intégral: The Korean Journal of Internal Medicine Année: 2018 Type: Article