Harmonization of laboratory results by data adjustment in multicenter clinical trials
The Korean Journal of Internal Medicine
; : 1119-1128, 2018.
Article
Dans En
| WPRIM
| ID: wpr-718020
Responsable en Bibliothèque :
WPRO
ABSTRACT
BACKGROUND/AIMS: In multicenter clinical trials, laboratory tests are performed in the laboratory of each center, mostly using different measuring methodologies. The purpose of this study was to evaluate coefficients of variation (CVs) of laboratory results produced by various measuring methods and to determine whether mathematical data adjustment could achieve harmonization between the methods. METHODS: We chose 10 clinical laboratories, including Green Cross Laboratories (GC Labs), the central laboratory, for the measurement of total cholesterol, high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), serum triglycerides, creatinine, and glucose. The serum panels made with patient samples referred to GC Labs were sent to the other laboratories. Twenty serum samples for each analyte were prepared, sent frozen, and analyzed by each participating laboratory. RESULTS: All methods used by participating laboratories for the six analytes had traceability by reference materials and methods. When the results from the nine laboratories were compared with those from GC Labs, the mean CVs for total cholesterol, HDL-C, LDL-C, and glucose analyzed using the same method were 1.7%, 3.7%, 4.3%, and 1.7%, respectively; and those for triglycerides and creatinine analyzed using two different methods were 4.5% and 4.48%, respectively. After adjusting data using Deming regression, the mean CV were 0.7%, 1.4%, 1.8%, 1.4%, 1.6%, and 0.8% for total cholesterol, HDL-C, LDL-C, triglyceride, creatinine, and glucose, respectively. CONCLUSIONS: We found that more comparable results can be produced by laboratory data harmonization using commutable samples. Therefore, harmonization efforts should be undertaken in multicenter trials for accurate data analysis (CRIS number; KCT0001235).
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Plan de recherche
/
Triglycéride
/
Cholestérol
/
Statistiques comme sujet
/
Études multicentriques comme sujet
/
Créatinine
/
Glucose
/
Cholestérol HDL
/
Cholestérol LDL
/
Méthodes
Type d'étude:
Clinical_trials
Limites du sujet:
Humans
langue:
En
Texte intégral:
The Korean Journal of Internal Medicine
Année:
2018
Type:
Article