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Removing Lipemia in Serum/Plasma Samples: A Multicenter Study
Annals of Laboratory Medicine ; : 518-523, 2018.
Article Dans Anglais | WPRIM | ID: wpr-718332
ABSTRACT

BACKGROUND:

Lipemia, a significant source of analytical errors in clinical laboratory settings, should be removed prior to measuring biochemical parameters. We investigated whether lipemia in serum/plasma samples can be removed using a method that is easier and more practicable than ultracentrifugation, the current reference method.

METHODS:

Seven hospital laboratories in Spain participated in this study. We first compared the effectiveness of ultracentrifugation (108,200×g) and high-speed centrifugation (10,000×g for 15 minutes) in removing lipemia. Second, we compared high-speed centrifugation with two liquid-liquid extraction methods—LipoClear (StatSpin, Norwood, USA), and 1,1,2-trichlorotrifluoroethane (Merck, Darmstadt, Germany). We assessed 14 biochemical parameters serum/plasma concentrations of sodium ion, potassium ion, chloride ion, glucose, total protein, albumin, creatinine, urea, alkaline phosphatase, gamma-glutamyl transferase, alanine aminotransferase, aspartate-aminotransferase, calcium, and bilirubin. We analyzed whether the differences between lipemia removal methods exceeded the limit for clinically significant interference (LCSI).

RESULTS:

When ultracentrifugation and high-speed centrifugation were compared, no parameter had a difference that exceeded the LCSI. When high-speed centrifugation was compared with the two liquid-liquid extraction methods, we found differences exceeding the LCSI in protein, calcium, and aspartate aminotransferase in the comparison with 1,1,2-trichlorotrifluoroethane, and in protein, albumin, and calcium in the comparison with LipoClear. Differences in other parameters did not exceed the LCSI.

CONCLUSIONS:

High-speed centrifugation (10,000×g for 15 minutes) can be used instead of ultracentrifugation to remove lipemia in serum/plasma samples. LipoClear and 1,1,2-trichlorotrifluoroethane are unsuitable as they interfere with the measurement of certain parameters.
Sujets)

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Potassium / Aspartate aminotransferases / Sodium / Espagne / Transferases / Ultracentrifugation / Urée / Bilirubine / Laboratoires hospitaliers / Centrifugation Type d'étude: Essai clinique contrôlé Pays comme sujet: Europe langue: Anglais Texte intégral: Annals of Laboratory Medicine Année: 2018 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Potassium / Aspartate aminotransferases / Sodium / Espagne / Transferases / Ultracentrifugation / Urée / Bilirubine / Laboratoires hospitaliers / Centrifugation Type d'étude: Essai clinique contrôlé Pays comme sujet: Europe langue: Anglais Texte intégral: Annals of Laboratory Medicine Année: 2018 Type: Article