Development and Evaluation of Hyaluronic Acid-Based Hybrid Bio-Ink for Tissue Regeneration
Tissue Engineering and Regenerative Medicine
;
(6): 761-769, 2018.
Article
Dans Anglais
| WPRIM
| ID: wpr-718789
ABSTRACT
BACKGROUND:
Bioprinting has recently appeared as a powerful tool for building complex tissue and organ structures. However, the application of bioprinting to regenerative medicine has limitations, due to the restricted choices of bio-ink for cytocompatible cell encapsulation and the integrity of the fabricated structures.METHODS:
In this study, we developed hybrid bio-inks based on acrylated hyaluronic acid (HA) for immobilizing bioactive peptides and tyramine-conjugated hyaluronic acids for fast gelation.RESULTS:
Conventional acrylated HA-based hydrogels have a gelation time of more than 30 min, whereas hybrid bioink has been rapidly gelated within 200 s. Fibroblast cells cultured in this hybrid bio-ink up to 7 days showed < 90% viability. As a guidance cue for stem cell differentiation, we immobilized four different bio-active peptides BMP-7-derived peptides (BMP-7D) and osteopontin for osteogenesis, and substance-P (SP) and Ac-SDKP (SDKP) for angiogenesis. Mesenchymal stem cells cultured in these hybrid bio-inks showed the highest angiogenic and osteogenic activity cultured in bio-ink immobilized with a SP or BMP-7D peptide. This bio-ink was loaded in a three-dimensional (3D) bioprinting device showing reproducible printing features.CONCLUSION:
We have developed bio-inks that combine biochemical and mechanical cues. Biochemical cues were able to regulate differentiation of cells, and mechanical cues enabled printing structuring. This multi-functional bio-ink can be used for complex tissue engineering and regenerative medicine.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Ostéogenèse
/
Peptides
/
Régénération
/
Cellules souches
/
Hydrogels
/
Signaux
/
Ingénierie tissulaire
/
Médecine régénérative
/
Ostéopontine
/
Cellules souches mésenchymateuses
langue:
Anglais
Texte intégral:
Tissue Engineering and Regenerative Medicine
Année:
2018
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS