Combined effect of recombinant human bone morphogenetic protein-2 and low level laser irradiation on bisphosphonate-treated osteoblasts
Journal of the Korean Association of Oral and Maxillofacial Surgeons
;
: 259-268, 2018.
Article
Dans Anglais
| WPRIM
| ID: wpr-718881
ABSTRACT
OBJECTIVES:
The purpose of this study was to evaluate the synergic effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) and low-level laser therapy (LLLT) on bisphosphonate-treated osteoblasts. MATERIALS ANDMETHODS:
Human fetal osteoblast cells (hFOB 1.19) were cultured with 100 µM alendronate. Low-level Ga-Al-As laser alone or with 100 ng/mL rhBMP-2 was then applied. Cell viability was measured with MTT assay. The expression levels of receptor activator of nuclear factor kappa-B ligand (RANKL), macrophage colony-stimulating factor (M-CSF), and osteoprotegerin (OPG) were analyzed for osteoblastic activity inducing osteoclastic activity. Collagen type and transforming growth factor beta-1 were also evaluated for bone matrix formation.RESULTS:
The results showed that rhBMP-2 and LLLT had a synergic effect on alendronate-treated osteoblasts for enhancing osteoblastic activity and bone matrix formation. Between rhBMP-2 and LLLT, rhBMP-2 exhibited a greater effect, but did not show a significant difference.CONCLUSION:
rhBMP-2 and LLLT have synergic effects on bisphosphonate-treated osteoblasts through enhancement of osteoblastic activity and bone formation activity.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Ostéoblastes
/
Ostéoclastes
/
Ostéogenèse
/
Trame osseuse
/
Facteurs de croissance transformants
/
Survie cellulaire
/
Facteur de stimulation des colonies de macrophages
/
Collagène
/
Alendronate
/
Photothérapie de faible intensité
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Journal of the Korean Association of Oral and Maxillofacial Surgeons
Année:
2018
Type:
Article
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