Enforced Expression of BMI-1 in Postnatal Human CD34+ Cells Promotes Erythroid Differentiation
Korean Journal of Hematology
; : 241-249, 2007.
Article
Dans En
| WPRIM
| ID: wpr-720789
Responsable en Bibliothèque :
WPRO
ABSTRACT
BACKGROUND: The Polycomb-group gene Bmi-1 is known to be a molecular regulator of self-renewal of normal and leukemic stem cells and be involved in various aspects of cellular proliferation, differentiation, and survival. METHODS: This study evaluated the effects of overexpression of Bmi-1 on human cord blood CD34+ cells. Bmi-1 was introduced into CD34+ cells through lentivirus transduction. Bmi-1 expressing CD34+ cells were applied to colony forming assay, stromal co-culture, and cytokine-stimulatied culture. RESULTS: Ectopic expression of Bmi-1 resulted in the increased number of erythroid colonies in primary and secondary colony forming assay in an erythropoietin dependent manner. In stromal co-culture, Bmi-1-expressing postnatal hematopoietic stem cells seemed to lose the ability of self-renewal, as determined by week 5 cobblestone area-forming cell assay and by week 5 secondary colony assay. In cytokine-stimulated suspension culture of Bmi-1-transduced CD34+ cells, we observed increased erythropoiesis marked by Glycophorin A expression. CONCLUSION: Our data suggest that ectopic expression of Bmi-1 in human hematopoietic stem/progenitor cells may result in the differentiation to the erythroid lineage rather than promoting self-renewal.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Cellules souches
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Glycophorines
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Cellules souches hématopoïétiques
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Érythropoïétine
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Lentivirus
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Techniques de coculture
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Prolifération cellulaire
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Érythropoïèse
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Sang foetal
Limites du sujet:
Humans
langue:
En
Texte intégral:
Korean Journal of Hematology
Année:
2007
Type:
Article