Rapid Detection of Extended Spectrum beta-Lactamase (ESBL) for Enterobacteriaceae by use of a Multiplex PCR-based Method / 감염과화학요법
Infection and Chemotherapy
;
: 181-184, 2009.
Article
Dans Anglais
| WPRIM
| ID: wpr-722121
ABSTRACT
A multiplex PCR method has been developed to classify extended spectrum beta-lactamase (ESBL) and plasmid-mediated AmpC beta-lactamase (PABL). This method consists of the use of two four-multiplex PCRs for the detection of TEM, OXA, SHV, CTX-M, CMY, and DHA type beta-lactamases. We have compared findings from the use of conventional detection methods with that of this newly developed typing method. In testing for 73 ESBL-producing and PABL-producing isolates, 100% of the isolates were correctly identified as previously characterized types and, 44 types of beta-lactamases were additionally identified from 33 isolates. This assay not only reduces the time for classification but also increases the accuracy for detection.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Protéines bactériennes
/
Bêta-Lactamases
/
Ocytocine
/
Réaction de polymérisation en chaîne
/
Enterobacteriaceae
/
Réaction de polymérisation en chaine multiplex
Type d'étude:
Etude diagnostique
/
Étude pronostique
langue:
Anglais
Texte intégral:
Infection and Chemotherapy
Année:
2009
Type:
Article
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