Effect of Hypoxia on the Melanogenesis of Murine B16 Melanoma Cells / 대한피부과학회지
Korean Journal of Dermatology
;
: 332-337, 2007.
Article
Dans Coréen
| WPRIM
| ID: wpr-72448
ABSTRACT
BACKGROUND:
Numerous reports suggest the role of oxygen in melanogenesis. However, little has been reported on the effect of a hypoxic environment on cellular melanogenesis.OBJECTIVE:
The effect of low oxygen tension on cellular melanogenesis was investigated in B16 murine melanoma cells.METHODS:
Using cells cultured under an ambient (21% O2) or hypoxic (5% O2) condition, melanin content and tyrosinase activity were measured spectrophotometrically. The expression of tyrosinase, tyrosinase-related protein (TRP)- 1, and TRP-2 were analyzed by RT-PCR and Western blot.RESULTS:
Culture of cells under hypoxic conditions caused significant inhibition of isobutylmethylxanthine (IBMX)- induced increase of melanin content. No cytotoxicity was observed during the hypoxic culture periods. Decreased melanin content occurred through the decrease of tyrosinase protein and activity (p<0.01). The mRNA levels of tyrosinase and TRP-2 were also decreased by hypoxia, while that of TRP-1 was unchanged. Similar inhibitions of melanin content and tyrosinase activity were observed in the cells stimulated with dibutyryl-cAMP.CONCLUSION:
IBMX-induced melanogenesis in B16 cells was significantly inhibited under hypoxic culture conditions, suggesting the important role of oxygen tension in cellular melanogenesis.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Oxygène
/
Mélanome expérimental
/
ARN messager
/
Technique de Western
/
Monophenol monooxygenase
/
Mélanines
/
Mélanome
/
Hypoxie
langue:
Coréen
Texte intégral:
Korean Journal of Dermatology
Année:
2007
Type:
Article
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