Activation of Transcription Factor NF-kappaB and c-Jun/AP-1 by Various Compositions of Particles in Osteoclast Precursor Cells
Journal of the Korean Hip Society
;
: 209-214, 2008.
Article
Dans Coréen
| WPRIM
| ID: wpr-727103
ABSTRACT
PURPOSE:
This study was performed in order to investigate the effects of various particle preparations on NF-kappaB and c-Jun/AP-1 activity in osteoclast precursor cells. MATERIALS ANDMETHODS:
Osteoclast precursor cells isolated from C57BL mice were treated with PMMA (polymethylmethacrylate) spheres, polystyrene, titanium particles, and retrieved metal particles from failed cementless total hip replacements. NF-kappaB and c-Jun/AP-1 DNA binding activities were analyzed using electrophoretic mobility shift assays (EMSA).RESULTS:
Commercially available PMMA and polystyrene spheres routinely showed negativity on endotoxin assays, but titanium particles and retrieved metal particles consistently showed positivity. PMMA spheres, with a maximal response noted at 30 minutes with an optimal concentration of 0.6 mg/ml, were potent stimulator of NF-kappaB and c-Jun/AP-1 activity in osteoclast precursor cells. Other particles (polystyrene, titanium, metal retrievals) also activated transcription factor NF-kappaB and c-Jun/AP-1 compared to controls. Endotoxin removal from retrieved metal particles diminished the biologic effect by approximately 40%.CONCLUSION:
Particles of various compositions and sizes (PMMA, polystyrene, titanium, and retrieved metal particles) activated the NF-kappaB and c-Jun/AP-1 signaling pathways. This suggests that NF-kappaB and c-Jun/AP-1 may have important roles in the pathogenesis of periprosthetic osteolysis.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Ostéoclastes
/
Ostéolyse
/
Polystyrènes
/
Titane
/
Facteurs de transcription
/
ADN
/
Facteur de transcription NF-kappa B
/
Poly(méthacrylate de méthyle)
/
Arthroplastie prothétique de hanche
/
Test de retard de migration électrophorétique
Limites du sujet:
Animaux
langue:
Coréen
Texte intégral:
Journal of the Korean Hip Society
Année:
2008
Type:
Article
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