Regulator of Calcineurin 1 Isoform 4 (RCAN1.4) Is Overexpressed in the Glomeruli of Diabetic Mice
The Korean Journal of Physiology and Pharmacology
; : 299-305, 2011.
Article
de En
| WPRIM
| ID: wpr-728327
Bibliothèque responsable:
WPRO
ABSTRACT
Calcineurin (CaN) is activated in diabetes and plays a role in glomerular hypertrophy and extracellular matrix (ECM) accumulation. Here, kidneys from diabetic model mice were investigated for the expression of the regulator of CaN 1 (RCAN1) isoform 4 (RCAN1.4) which had been shown to be transcriptionally upregulated by CaN activation. We found the increased immunoreactivity for RCAN1 in the glomerular cells of db/db mice and streptozotocin-induced diabetic mice. In concordance, the expression of RCAN1 protein and RCAN1.4 mRNA were elevated in the whole kidney sample from db/db mice. Interleukin-1beta (IL-1beta), tumor necrosis factor-alpha, and glycated albumin (AGE-BSA) were identified as inducers of RCAN1.4 in mesangial cells. Pretreatment of cyclosporine A blocked the increases of RCAN1.4 stimulated by IL-1beta or AGE-BSA, suggesting that activation of CaN is required for the RCAN1.4 induction. Stable transfection of RCAN1.4 in Mes-13 mesangial cells upregulated several factors relevant to ECM production and degradation. These results suggested that RCAN1.4 might act as a link between CaN activation and ECM turnover in diabetic nephropathy.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
ARN messager
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Sérumalbumine
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Sérumalbumine bovine
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Carbonates
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Transfection
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Facteur de nécrose tumorale alpha
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Ciclosporine
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Calcineurine
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Néphropathies diabétiques
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Cellules mésangiales
Type d'étude:
Prognostic_studies
Limites du sujet:
Animals
langue:
En
Texte intégral:
The Korean Journal of Physiology and Pharmacology
Année:
2011
Type:
Article