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Protein Kinase C-mediated Neuroprotective Action of (-)-epigallocatechin-3-gallate against Abeta1-42-induced Apoptotic Cell Death in SH-SY5Y Neuroblastoma Cells
The Korean Journal of Physiology and Pharmacology ; : 163-169, 2007.
Article Dans Anglais | WPRIM | ID: wpr-728471
ABSTRACT
The neurotoxicity of amyloid beta (Abeta) is associated with an increased production of reactive oxygen species and apoptosis, and it has been implicated in the development of Alzheimer's disease. While (-)-epigallocatechin-3-gallate (EGCG) suppresses Abeta-induced apoptosis, the mechanisms underlying this process have yet to be completely clarified. This study was designed to investigate whether EGCG plays a neuroprotective role by activating cell survival system such as protein kinase C (PKC), extracellular-signal-related kinase (ERK), c-Jun N-terminal kinase (JNK), and anti-apoptotic and pro-apoptotic genes in SH-SY5Y human neuroblastoma cells. One microM Abeta1-42 decreased cell viability, which was correlated with increased DNA fragmentation evidenced by DAPI staining. Pre-treatment of SH-SY5Y neuroblastoma cells with EGCG (1microM) significantly attenuated Abeta1-42-induced cytotoxicity. Potential cell signaling candidates involved in this neuroprotective effects were further examined. EGCG restored the reduced PKC, ERK, and JNK activities caused by Abeta1-42 toxicity. In addition, gene expression analysis revealed that EGCG prevented both the Abeta1-42-induced expression of a pro-apoptotic gene mRNA, Bad and Bax, and the decrease of an anti-apoptotic gene mRNA, Bcl-2 and Bcl-xl. These results suggest that the neuroprotective mechanism of EGCG against Abeta1-42-induced apoptotic cell death includes stimulation of PKC, ERK, and JNK, and modulation of cell survival and death genes.
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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Phosphotransferases / Protein kinases / Protéine kinase C / ARN messager / Expression des gènes / Survie cellulaire / Mort cellulaire / Espèces réactives de l'oxygène / Apoptose / Neuroprotecteurs Limites du sujet: Humains langue: Anglais Texte intégral: The Korean Journal of Physiology and Pharmacology Année: 2007 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Phosphotransferases / Protein kinases / Protéine kinase C / ARN messager / Expression des gènes / Survie cellulaire / Mort cellulaire / Espèces réactives de l'oxygène / Apoptose / Neuroprotecteurs Limites du sujet: Humains langue: Anglais Texte intégral: The Korean Journal of Physiology and Pharmacology Année: 2007 Type: Article