Improvement of Fungal Cellulase Production by Mutation and Optimization of Solid State Fermentation
Mycobiology
;
: 20-25, 2011.
Article
Dans Anglais
| WPRIM
| ID: wpr-729253
ABSTRACT
Spores of Aspergillus sp. SU14 were treated repeatedly and sequentially with Co60 gamma-rays, ultraviolet irradiation, and N-methyl-N'-nitro-N-nitrosoguanidine. One selected mutant strain, Aspergillus sp. SU14-M15, produced cellulase in a yield 2.2-fold exceeding that of the wild type. Optimal conditions for the production of cellulase by the mutant fungal strain using solid-state fermentation were examined. The medium consisted of wheat-bran supplemented with 1% (w/w) urea or NH4Cl, 1% (w/w) rice starch, 2.5 mM MgCl2, and 0.05% (v/w) Tween 80. Optimal moisture content and initial pH was 50% (v/w) and 3.5, respectively, and optimal aeration area was 3/100 (inoculated wheat bran/container). The medium was inoculated with 25% 48 hr seeding culture and fermented at 35degrees C for 3 days. The resulting cellulase yield was 8.5-fold more than that of the wild type strain grown on the basal wheat bran medium.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Polysorbates
/
Aspergillus
/
Graines
/
Spores
/
Entorses et foulures
/
Amidon
/
Urée
/
Triticum
/
Fibre alimentaire
/
Chlorure de magnésium
langue:
Anglais
Texte intégral:
Mycobiology
Année:
2011
Type:
Article
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