Genomic Organization of ancop Gene for alpha-COP Homolog from Aspergillus nidulans
Mycobiology
;
: 171-176, 2000.
Article
Dans Anglais
| WPRIM
| ID: wpr-729318
ABSTRACT
We have cloned a alpha-COP homolog, ancop, from Aspergillus nidulans by colony hybridization of chromosome specific library using alpha-COP homologous fragment as a probe. The probe DNA was amplified with degenerated primers designed by comparison of conserved region of the amino acid sequences of Saccharomyces cerevisiae alpha-COP, Homo sapiens HEP-COP, and Drosophila melanogaster alpha-COP. Full length cDNA clone was also amplified by RT-PCR. Comparison of genomic DNA sequence with cDNA sequence obtained by RT-PCR revealed 7 introns. Amino acid sequence similarity search of the anCop with other alpha-COPs gave an overall identity of 52% with S. cerevisiae, 47% with human and bovine, 45% with Drosophila and Arabidopsis . In upstream region from the transcription start site, a putative TATA and CAAT motif were also identified.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Aspergillus
/
Aspergillus nidulans
/
Saccharomyces cerevisiae
/
ADN
/
Séquence nucléotidique
/
Introns
/
Séquence d'acides aminés
/
Clones cellulaires
/
Similitude de séquences
/
Arabidopsis
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Mycobiology
Année:
2000
Type:
Article
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