Generation and Evaluation of High beta-Glucan Producing Mutant Strains of Sparassis crispa
Mycobiology
;
: 159-163, 2013.
Article
Dans Anglais
| WPRIM
| ID: wpr-729419
ABSTRACT
A chemical mutagenesis technique was employed for development of mutant strains of Sparassis crispa targeting the shortened cultivation time and the high beta-glucan content. The homogenized mycelial fragments of S. crispa IUM4010 strain were treated with 0.2 vol% methyl methanesulfonate, an alkylating agent, yielding 199 mutant strains. Subsequent screening in terms of growth and beta-glucan content yielded two mutant strains, B4 and S7. Both mutants exhibited a significant increase in beta-glucan productivity by producing 0.254 and 0.236 mg soluble beta-glucan/mg dry cell weight for the B4 and S7 strains, respectively, whereas the wild type strain produced 0.102 mg soluble beta-glucan/mg dry cell weight. The results demonstrate the usefulness of chemical mutagenesis for generation of mutant mushroom strains.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Entorses et foulures
/
Dépistage de masse
/
Mutagenèse
/
Méthanesulfonates
/
Agaricales
/
Rendement
/
Méthanesulfonate de méthyle
Type d'étude:
Étude de dépistage
langue:
Anglais
Texte intégral:
Mycobiology
Année:
2013
Type:
Article
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