Development of Detection Methods for Cellulolytic Activity of Auricularia auricula-judae
Mycobiology
;
: 74-77, 2010.
Article
Dans Anglais
| WPRIM
| ID: wpr-729543
ABSTRACT
To obtain basic information on the detection of cellulolytic activity in Auricularia auricula-judae, the influences of dye reagent, pH, and temperature were assessed. Chromogenic dye (congo red, phenol red, remazol brilliant blue, and trypan blue) was individually incorporated into a medium containing either carboxymethyl-cellulose, Avicel, or D-cellobiose as a polysaccharide carbon substrate. The other assessments utilized pHs ranging from 4.5 to 8.0 and temperatures from 15~35degrees C. Overall, when A. auricula-judae species were transferred onto media contained Congo red and adjusted pH 7.0 and then incubated at 25degrees C for 5 days, the clear zone indicative of cellulolytic activity was more pronounced.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phénolsulfonephtaléine
/
Benzènesulfonates
/
Carbone
/
Cellulose
/
Rouge Congo
/
Diminazène
/
Concentration en ions d'hydrogène
Type d'étude:
Etude diagnostique
langue:
Anglais
Texte intégral:
Mycobiology
Année:
2010
Type:
Article
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