Heterologous Expression of Phanerochaete chrysoporium Glyoxal Oxidase and its Application for the Coupled Reaction with Manganese Peroxidase to Decolorize Malachite Green
Mycobiology
;
: 258-262, 2012.
Article
Dans Anglais
| WPRIM
| ID: wpr-729695
ABSTRACT
cDNA of the glx1 gene encoding glyoxal oxidase (GLX) from Phanerochaete chrysosporium was isolated and expressed in Pichia pastoris. The recombinant GLX (rGLX) produces H2O2 over 7.0 nmol/min/mL using methyl glyoxal as a substrate. Use of rGLX as a generator of H2O2 improved the coupled reaction with recombinant manganese peroxidase resulting in decolorization of malachite green up to 150 microM within 90 min.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Composés organométalliques
/
Oxidoreductases
/
Peroxidases
/
Pichia
/
Magenta I
/
ADN complémentaire
/
Myeloperoxidase
/
Phanerochaete
/
Alcohol oxidoreductases
/
Glyoxal
langue:
Anglais
Texte intégral:
Mycobiology
Année:
2012
Type:
Article
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